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Genes on the move: in vitro transduction of antimicrobial resistance genes between human and canine staphylococcal pathogens

Frosini, SM; Bond, R; McCarthy, AJ; Feudi, C; Schwarz, S; Lindsay, JA; Loeffler, A (2020) Genes on the move: in vitro transduction of antimicrobial resistance genes between human and canine staphylococcal pathogens. Microorganisms, 8 (12). p. 2031. ISSN 2076-2607 https://doi.org/10.3390/microorganisms8122031
SGUL Authors: Lindsay, Jodi Anne

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Abstract

Transmission of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) between people and pets, and their co-carriage, are well-described. Potential exchange of antimicrobial resistance (AMR) genes amongst these staphylococci was investigated in vitro through endogenous bacteriophage-mediated transduction. Bacteriophages were UV-induced from seven donor isolates of canine (MRSP) and human (MRSA) origin, containing tet(M), tet(K), fusB or fusC, and lysates filtered. Twenty-seven tetracycline- and fusidic acid- (FA-) susceptible recipients were used in 122 donor-recipient combinations (22 tetracycline, 100 FA) across 415 assays (115 tetracycline, 300 FA). Bacteriophage lysates were incubated with recipients and presumed transductants quantified on antimicrobial-supplemented agar plates. Tetracycline resistance transduction from MRSP and MRSA to methicillin-susceptible S. pseudintermedius (MSSP) was confirmed by PCR in 15/115 assays. No FA-resistance transfer occurred, confirmed by negative fusB/fusC PCR, but colonies resulting from FA assays had high MICs (≥32 mg/L) and showed mutations in fusA, two at a novel position (F88L), nine at H457[Y/N/L]. Horizontal gene transfer of tetracycline-resistance confirms that resistance genes can be shared between coagulase-positive staphylococci from different hosts. Cross-species AMR transmission highlights the importance of good antimicrobial stewardship across humans and veterinary species to support One Health.

Item Type: Article
Additional Information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Microorganisms
ISSN: 2076-2607
Dates:
DateEvent
18 December 2020Published
17 December 2020Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
BB/K011952/1Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
FKZ 01KI1707Bundesministerium für Bildung und ForschungUNSPECIFIED
URI: https://openaccess.sgul.ac.uk/id/eprint/112725
Publisher's version: https://doi.org/10.3390/microorganisms8122031

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