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Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia.

Morales-Yánez, F; Trashin, S; Sariego, I; Roucher, C; Paredis, L; Chico, M; De Wael, K; Muyldermans, S; Cooper, P; Polman, K (2020) Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia. Parasit Vectors, 13 (1). p. 245. ISSN 1756-3305 https://doi.org/10.1186/s13071-020-04113-2
SGUL Authors: Cooper, Philip John

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Abstract

BACKGROUND: The diagnosis of active Toxocara canis infections in humans is challenging. Larval stages of T. canis do not replicate in human tissues and disease may result from infection with a single T. canis larva. Recently, we developed a nanobody-based electrochemical magnetosensor assay with superior sensitivity to detect T. canis excretory-secretory (TES) antigens. Here, we evaluate the performance of the assay in children from an Ecuadorian birth cohort that followed children to five years of age. METHODS: Samples were selected based on the presence of peripheral blood eosinophilia and relative eosinophil counts. The samples were analyzed by the nanobody-based electrochemical magnetosensor assay, which utilizes a bivalent biotinylated nanobody as capturing agent on the surface of streptavidin pre-coated paramagnetic beads. Detection was performed by a different nanobody chemically labelled with horseradish peroxidase. RESULTS: Of 87 samples tested, 33 (38%) scored positive for TES antigen recognition by the electrochemical magnetosensor assay. The average concentration of TES antigen in serum was 2.1 ng/ml (SD = 1.1). The positive result in the electrochemical assay was associated with eosinophilia > 19% (P = 0.001). Parasitological data were available for 57 samples. There was no significant association between positivity by the electrochemical assay and the presence of other soil-transmitted helminth infections. CONCLUSIONS: Our nanobody-based electrochemical assay provides highly sensitive quantification of TES antigens in serum and has potential as a valuable tool for the diagnosis of active human toxocariasis.

Item Type: Article
Additional Information: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Keywords: Electrochemical assay, Eosinophilia, Nanobodies, Toxocara, 1108 Medical Microbiology, 1117 Public Health and Health Services, Tropical Medicine, Mycology & Parasitology
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Parasit Vectors
ISSN: 1756-3305
Language: eng
Dates:
DateEvent
12 May 2020Published
29 April 2020Accepted
Projects:
Project IDFunderFunder ID
G.0189.13NFonds Wetenschappelijk OnderzoekUNSPECIFIED
072405/Z/03/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
088862/Z/09/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
PubMed ID: 32398157
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/111955
Publisher's version: https://doi.org/10.1186/s13071-020-04113-2

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