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Maternal Larp6 controls oocyte development, chorion formation and elevation.

Hau, HTA; Ogundele, O; Hibbert, AH; Monfries, CAL; Exelby, K; Wood, NJ; Nevarez-Mejia, J; Carbajal, MA; Fleck, RA; Dermit, M; et al. Hau, HTA; Ogundele, O; Hibbert, AH; Monfries, CAL; Exelby, K; Wood, NJ; Nevarez-Mejia, J; Carbajal, MA; Fleck, RA; Dermit, M; Mardakheh, FK; Williams-Ward, VC; Pipalia, TG; Conte, MR; Hughes, SM (2020) Maternal Larp6 controls oocyte development, chorion formation and elevation. Development, 147 (4). ISSN 1477-9129 https://doi.org/10.1242/dev.187385
SGUL Authors: Monfries, Clinton Alphaeus Lloyd

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Abstract

La-related protein 6 (Larp6) is a conserved RNA-binding protein found across eukaryotes that has been suggested to regulate collagen biogenesis, muscle development, ciliogenesis, and various aspects of cell proliferation and migration. Zebrafish have two Larp6 family genes: larp6a and larp6b Viable and fertile single and double homozygous larp6a and larp6b zygotic mutants revealed no defects in muscle structure, and were indistinguishable from heterozygous or wild-type siblings. However, larp6a mutant females produced eggs with chorions that failed to elevate fully and were fragile. Eggs from larp6b single mutant females showed minor chorion defects, but chorions from eggs laid by larp6a;larp6b double mutant females were more defective than those from larp6a single mutants. Electron microscopy revealed defective chorionogenesis during oocyte development. Despite this, maternal zygotic single and double mutants were viable and fertile. Mass spectrometry analysis provided a description of chorion protein composition and revealed significant reductions in a subset of zona pellucida and lectin-type proteins between wild-type and mutant chorions that paralleled the severity of the phenotype. We conclude that Larp6 proteins are required for normal oocyte development, chorion formation and egg activation.

Item Type: Article
Additional Information: © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Keywords: Chorion, Knockout, Larp6, Mass spectrometry, Maternal effect, Oocyte, Zebrafish, Zebrafish, Larp6, Knockout, Oocyte, Chorion, Mass spectrometry, Maternal effect, 06 Biological Sciences, 11 Medical and Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: Development
ISSN: 1477-9129
Language: eng
Dates:
DateEvent
26 February 2020Published
23 January 2020Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
093389/Z/10/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
MR/N021231/1Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
MR/P009417/1Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
T37 MD001485NIMHD NIH HHSUNSPECIFIED
G1001029Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
PubMed ID: 32054660
Web of Science ID: WOS:000522794700022
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/111928
Publisher's version: https://doi.org/10.1242/dev.187385

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