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Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma.

Waseem, NH; Low, S; Shah, AZ; Avisetti, D; Ostergaard, P; Simpson, M; Niemiec, KA; Martin-Martin, B; Aldehlawi, H; Usman, S; et al. Waseem, NH; Low, S; Shah, AZ; Avisetti, D; Ostergaard, P; Simpson, M; Niemiec, KA; Martin-Martin, B; Aldehlawi, H; Usman, S; Lee, PS; Khawaja, AP; Ruddle, JB; Shah, A; Sackey, E; Day, A; Jiang, Y; Swinfield, G; Viswanathan, A; Alfano, G; Chakarova, C; Cordell, HJ; Garway-Heath, DF; Khaw, PT; Bhattacharya, SS; Waseem, A; Foster, PJ (2020) Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma. PLoS Genet, 16 (4). e1008721. ISSN 1553-7404 https://doi.org/10.1371/journal.pgen.1008721
SGUL Authors: Ostergaard, Pia

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Abstract

Current estimates suggest 50% of glaucoma blindness worldwide is caused by primary angle-closure glaucoma (PACG) but the causative gene is not known. We used genetic linkage and whole genome sequencing to identify Spermatogenesis Associated Protein 13, SPATA13 (NM_001166271; NP_001159743, SPATA13 isoform I), also known as ASEF2 (Adenomatous polyposis coli-stimulated guanine nucleotide exchange factor 2), as the causal gene for PACG in a large seven-generation white British family showing variable expression and incomplete penetrance. The 9 bp deletion, c.1432_1440del; p.478_480del was present in all affected individuals with angle-closure disease. We show ubiquitous expression of this transcript in cell lines derived from human tissues and in iris, retina, retinal pigment and ciliary epithelia, cornea and lens. We also identified eight additional mutations in SPATA13 in a cohort of 189 unrelated PACS/PAC/PACG samples. This gene encodes a 1277 residue protein which localises to the nucleus with partial co-localisation with nuclear speckles. In cells undergoing mitosis SPATA13 isoform I becomes part of the kinetochore complex co-localising with two kinetochore markers, polo like kinase 1 (PLK-1) and centrosome-associated protein E (CENP-E). The 9 bp deletion reported in this study increases the RAC1-dependent guanine nucleotide exchange factors (GEF) activity. The increase in GEF activity was also observed in three other variants identified in this study. Taken together, our data suggest that SPATA13 is involved in the regulation of mitosis and the mutations dysregulate GEF activity affecting homeostasis in tissues where it is highly expressed, influencing PACG pathogenesis.

Item Type: Article
Additional Information: Copyright: © 2020 Waseem et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: 0604 Genetics, Developmental Biology
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Journal or Publication Title: PLoS Genet
ISSN: 1553-7404
Language: eng
Dates:
DateEvent
27 April 2020Published
17 March 2020Accepted
Publisher License: Creative Commons: Attribution 4.0
PubMed ID: 32339198
Web of Science ID: WOS:000531363700012
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/111881
Publisher's version: https://doi.org/10.1371/journal.pgen.1008721

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