Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

Testicular expression of TDRD1, TDRD5, TDRD9 and TDRD12 in azoospermia.

Babakhanzadeh, E; Khodadadian, A; Rostami, S; Alipourfard, I; Aghaei, M; Nazari, M; Hosseinnia, M; Mehrjardi, MYV; Jamshidi, Y; Ghasemi, N (2020) Testicular expression of TDRD1, TDRD5, TDRD9 and TDRD12 in azoospermia. BMC Med Genet, 21 (1). p. 33. ISSN 1471-2350
SGUL Authors: Jamshidi, Yalda

PDF Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview


BACKGROUND: Tudor domain-containing proteins (TDRDs) play a critical role in piRNA biogenesis and germ cell development. piRNAs, small regulatory RNAs, act by silencing of transposons during germline development and it has recently been shown in animal model studies that defects in TDRD genes can lead to sterility in males. METHODS: Here we evaluate gene and protein expression levels of four key TDRDs (TDRD1, TDRD5, TDRD9 and TDRD12) in testicular biopsy samples obtained from men with obstructive azoospermia (OA, n = 29), as controls, and various types of non-obstructive azoospermia containing hypospermatogenesis (HP, 28), maturation arrest (MA, n = 30), and Sertoli cell-only syndrome (SCOS, n = 32) as cases. One-way ANOVA test followed by Dunnett's multiple comparison post-test was used to determine inter-group differences in TDRD gene expression among cases and controls. RESULTS: The results showed very low expression of TDRD genes in SCOS specimens. Also, the expression of TDRD1 and TDRD9 genes were lower in MA samples compared to OA samples. The expression of TDRD5 significantly reduced in SCOS, MA and HP specimens than the OA specimens. Indeed, TDRD12 exhibited a very low expression in HP specimens in comparison to OA specimens. All these results were confirmed by Western blot technique. CONCLUSION: TDRDs could be very important in male infertility, which should be express in certain stages of spermatogenesis.

Item Type: Article
Additional Information: © The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.
Keywords: Non-obstructive Azoospermia, Spermatogenesis, TDRD genes, piRNAs, Adult, Animals, Azoospermia, Cell Cycle Proteins, DNA Helicases, Gene Expression Regulation, Humans, Infertility, Male, Male, RNA, Small Interfering, Spermatogenesis, Testis, 0604 Genetics, 1103 Clinical Sciences, Genetics & Heredity
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Journal or Publication Title: BMC Med Genet
ISSN: 1471-2350
Language: eng
14 February 2020Published
10 February 2020Accepted
Publisher License: Creative Commons: Attribution 4.0
PubMed ID: 32059713
Go to PubMed abstract
Publisher's version:

Actions (login required)

Edit Item Edit Item