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Ability of SP12 mutant of S. typhi to effectively induce antibody responses to the mucosal antigen enterotoxigenic E. coli heat labile toxin B subunit after oral delivery to humans

Khan, S; Chatfield, S; Stratford, R; Bedwell, J; Bentley, M; Sulsh, S; Giemza, R; Smith, S; Bongard, E; Cosgrove, CA; et al. Khan, S; Chatfield, S; Stratford, R; Bedwell, J; Bentley, M; Sulsh, S; Giemza, R; Smith, S; Bongard, E; Cosgrove, CA; Johnson, J; Dougan, G; Griffin, GE; Makin, J; Lewis, DJ (2007) Ability of SP12 mutant of S. typhi to effectively induce antibody responses to the mucosal antigen enterotoxigenic E. coli heat labile toxin B subunit after oral delivery to humans. VACCINE, 25 (21). 4175 - 4182. ISSN 0264-410X https://doi.org/10.1016/j.vaccine.2007.03.007
SGUL Authors: Griffin, George Edward Lewis, David John Murdoch

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Abstract

We have evaluated an oral vaccine based on an Salmonella enteric serovar typhi (S. typhi) Ty2 derivative TSB7 harboring deletion mutations in ssaV (SPI-2) and aroC together with a chromosomally integrated copy of eltB encoding the B subunit of enterotoxigenic Escherichia coli heat labile toxin (LT-B) in volunteers. Two oral doses of 108 or 109 CFU were administered to two groups of volunteers and both doses were well tolerated, with no vaccinemia, and only transient stool shedding. Immune responses to LT-B and S. typhi lipopolysaccharide were demonstrated in 67 and 97% of subjects, respectively, without evidence of anti-carrier immunity preventing boosting of LT-B responses in many cases. Further development of this salmonella-based (spi-VEC) system for oral delivery of heterologous antigens appears warranted.

Item Type: Article
Additional Information: © 2007 Elsevier Ltd. Published under a Creative Commons license (CC BY 3.0).
Keywords: Administration, Oral, Adolescent, Adult, Antibodies, Bacterial, Antitoxins, Bacterial Toxins, Bacterial Vaccines, Blood, Enterotoxins, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Escherichia coli Proteins, Feces, Humans, Immunoglobulin G, Lymphocytes, Middle Aged, Protein Subunits, Salmonella typhi, Vaccines, Attenuated, Science & Technology, Life Sciences & Biomedicine, Immunology, Medicine, Research & Experimental, Research & Experimental Medicine, typhoid, salmonella, oral vaccine, ETEC, enterotoxigenic E. coli, CELL CHOLERA VACCINE, III SECRETION SYSTEM, ESCHERICHIA-COLI, SALMONELLA-TYPHIMURIUM, COLONIZATION FACTORS, HELICOBACTER-PYLORI, EXPRESSING UREASE, HUMAN VOLUNTEERS, IMMUNOGENICITY, DERIVATIVES
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: VACCINE
ISSN: 0264-410X
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Dates:
DateEvent
22 May 2007Published
Web of Science ID: WOS:000247640900008
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URI: http://openaccess.sgul.ac.uk/id/eprint/1117
Publisher's version: https://doi.org/10.1016/j.vaccine.2007.03.007

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