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Compensatory growth renders Tcf7l1a dispensable for eye formation despite its requirement in eye field specification

Young, RM; Hawkins, TA; Cavodeassi, F; Stickney, HL; Schwarz, Q; Lawrence, LM; Wierzbicki, C; Cheng, BYL; Luo, J; Ambrosio, EM; et al. Young, RM; Hawkins, TA; Cavodeassi, F; Stickney, HL; Schwarz, Q; Lawrence, LM; Wierzbicki, C; Cheng, BYL; Luo, J; Ambrosio, EM; Klosner, A; Sealy, IM; Rowell, J; Trivedi, CA; Bianco, IH; Allende, ML; Busch-Nentwich, EM; Gestri, G; Wilson, SW (2019) Compensatory growth renders Tcf7l1a dispensable for eye formation despite its requirement in eye field specification. eLife, 8. e40093. ISSN 2050-084X https://doi.org/10.7554/eLife.40093
SGUL Authors: Cavodeassi, Florencia

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Abstract

The vertebrate eye originates from the eye field, a domain of cells specified by a small number of transcription factors. In this study, we show that Tcf7l1a is one such transcription factor that acts cell-autonomously to specify the eye field in zebrafish. Despite the much-reduced eye field in tcf7l1a mutants, these fish develop normal eyes revealing a striking ability of the eye to recover from a severe early phenotype. This robustness is not mediated through genetic compensation at neural plate stage; instead, the smaller optic vesicle of tcf7l1a mutants shows delayed neurogenesis and continues to grow until it achieves approximately normal size. Although the developing eye is robust to the lack of Tcf7l1a function, it is sensitised to the effects of additional mutations. In support of this, a forward genetic screen identified mutations in hesx1, cct5 and gdf6a, which give synthetically enhanced eye specification or growth phenotypes when in combination with the tcf7l1a mutation.

Item Type: Article
Additional Information: © 2019, Young et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: eLife
ISSN: 2050-084X
Dates:
DateEvent
19 February 2019Published Online
26 January 2019Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
088175/Z/09/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
UNSPECIFIEDHorizon 2020UNSPECIFIED
UNSPECIFIEDRoyal Societyhttp://dx.doi.org/10.13039/501100000288
15090007Fondo de Financiamiento de Centros de Investigación en Áreas PrioritariasUNSPECIFIED
WT098051Wellcome Trusthttp://dx.doi.org/10.13039/100004440
206194Wellcome Trusthttp://dx.doi.org/10.13039/100004440
MR/L003775/1Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
104682/Z/14/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
089227/Z09/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
URI: http://openaccess.sgul.ac.uk/id/eprint/110673
Publisher's version: https://doi.org/10.7554/eLife.40093

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