SORA

Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus.

To, K-N; Cornwell, E; Daniel, R; Goonesekera, S; Jauneikaite, E; Chalker, V; Le Doare, K (2019) Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus. BMC Res Notes, 12 (1). p. 85. ISSN 1756-0500 https://doi.org/10.1186/s13104-019-4119-1
SGUL Authors: Le Doare, Kirsty

[img]
Preview
PDF Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
[img] Microsoft Word (.docx) Accepted Version
Restricted to Repository staff only
Available under License Creative Commons Attribution.

Download (1MB)

Abstract

Objective Group B Streptococcus (GBS) is a leading cause of neonatal meningitis and sepsis worldwide. Intrapartum antibiotics given to women carrying GBS are an effective means of reducing disease in the first week of life. Rapid and reliable tests are needed to accurately identify GBS from these women for timely intrapartum antibiotic administration to prevent neonatal disease. Many laboratories now use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) by direct plating or cell lysis for the identification of GBS isolates. The cell lysis step increases time to results for clinical samples and is more complex to perform. Therefore, we seek to evaluate the sensitivity and specificity of the quicker and more rapid direct plating method in identifying GBS. Results We directly compared swab isolates analysed by both direct plating and cell lysis method and demonstrated that direct plating has a sensitivity and specificity of 0.97 and 1, respectively, compared to an additional cell lysis step. We demonstrated that MALDI-TOF MS can be successfully used for batch processing by the direct plating method which saves time. These results are reassuring for laboratories worldwide who seek to identify GBS from swabs samples as quickly as possible.

Item Type: Article
Additional Information: © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Keywords: Group B Streptococcus, MALDI-TOF MS, Rapid diagnostics, Streptococcus agalactiae, 1199 Other Medical And Health Sciences, Bioinformatics
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: BMC Res Notes
ISSN: 1756-0500
Language: eng
Dates:
DateEvent
14 February 2019Published
6 February 2019Accepted
Projects:
Project IDFunderFunder ID
OPP1153630Bill and Melinda Gates Foundationhttp://dx.doi.org/10.13039/100000865
14485Thrasher Research Fundhttp://dx.doi.org/10.13039/100005627
PubMed ID: 30764872
Go to PubMed abstract
URI: http://openaccess.sgul.ac.uk/id/eprint/110601
Publisher's version: https://doi.org/10.1186/s13104-019-4119-1

Actions (login required)

Edit Item Edit Item