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Analysis of historical negative control group data from the in vitro micronucleus assay using human lymphocytes.

Lovell, DP; Fellow, M; Elhajouji, A; Farabaugh, CS; Gilby, BG; Hashimoto, K; Li, Y; Roy, S; Schuler, M; Whitwell, J; et al. Lovell, DP; Fellow, M; Elhajouji, A; Farabaugh, CS; Gilby, BG; Hashimoto, K; Li, Y; Roy, S; Schuler, M; Whitwell, J; Tanir, JY (2019) Analysis of historical negative control group data from the in vitro micronucleus assay using human lymphocytes. Mutat Res, 837. pp. 52-59. ISSN 1873-135X https://doi.org/10.1016/j.mrgentox.2018.08.009
SGUL Authors: Lovell, David

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Abstract

A database of the micronuclei counts was built up for historical negative control data from human lymphocyte in vitro micronuclei tests (MnVit) carried out in 8 laboratories with experience of the method. The mean incidence of micronucleated cells (mnt)/1000 cells ranged from 2.2/1000 to 15.9/1000. There were no large differences in incidence between the presence or absence of S9 mix or between different treatment lengths. There was also little evidence that different solvents affected the numbers of micronuclei appreciably. A number of laboratories did show significant inter-experiment variability, indicating that there remained unidentified factors affecting frequencies. Donor variance may be one such factor. Inter-individual variability may explain some of these differences. The approximate 7.5-fold difference in mnt/1000 scores in a relatively small group of experienced laboratories illustrates the potential complications that can arise if a metric like a fold increase was considered the only biologically important finding. Although there is inherent variability between experiments, it was evident that within a laboratory the overall laboratory mean remains constant over time. It is believed that these findings will provide help to laboratories conducting studies using human lymphocytes in the MnVit and to those involved in the assessment of MnVit results.

Item Type: Article
Additional Information: © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Keywords: Historical negative control data, Human lymphocytes, In vitro micronucleus assay, Quality control statistics, Oncology & Carcinogenesis
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: Mutat Res
ISSN: 1873-135X
Language: eng
Dates:
DateEvent
January 2019Published
18 September 2018Published Online
24 August 2018Accepted
Publisher License: Creative Commons: Attribution 4.0
PubMed ID: 30595210
Go to PubMed abstract
URI: http://openaccess.sgul.ac.uk/id/eprint/110519
Publisher's version: https://doi.org/10.1016/j.mrgentox.2018.08.009

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