SORA

Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

Opposing Shh and Fgf signals initiate nasotemporal patterning of the zebrafish retina.

Hernández-Bejarano, M; Gestri, G; Spawls, L; Nieto-López, F; Picker, A; Tada, M; Brand, M; Bovolenta, P; Wilson, SW; Cavodeassi, F (2015) Opposing Shh and Fgf signals initiate nasotemporal patterning of the zebrafish retina. Development, 142 (22). pp. 3933-3942. ISSN 1477-9129 https://doi.org/10.1242/dev.125120
SGUL Authors: Cavodeassi, Florencia

[img]
Preview
PDF Published Version
Available under License Creative Commons Attribution.

Download (3MB) | Preview

Abstract

The earliest known determinants of retinal nasotemporal identity are the transcriptional regulators Foxg1, which is expressed in the prospective nasal optic vesicle, and Foxd1, which is expressed in the prospective temporal optic vesicle. Previous work has shown that, in zebrafish, Fgf signals from the dorsal forebrain and olfactory primordia are required to specify nasal identity in the dorsal, prospective nasal, optic vesicle. Here, we show that Hh signalling from the ventral forebrain is required for specification of temporal identity in the ventral optic vesicle and is sufficient to induce temporal character when activated in the prospective nasal retina. Consequently, the evaginating optic vesicles become partitioned into prospective nasal and temporal domains by the opposing actions of Fgfs and Shh emanating from dorsal and ventral domains of the forebrain primordium. In absence of Fgf activity, foxd1 expression is established irrespective of levels of Hh signalling, indicating that the role of Shh in promoting foxd1 expression is only required in the presence of Fgf activity. Once the spatially complementary expression of foxd1 and foxg1 is established, the boundary between expression domains is maintained by mutual repression between Foxd1 and Foxg1.

Item Type: Article
Additional Information: © 2015. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Keywords: Fgfs, Nasotemporal patterning, Retina, Shh, Zebrafish, Animals, Body Patterning, Carbocyanines, Fibroblast Growth Factors, Forkhead Transcription Factors, Hedgehog Proteins, Image Processing, Computer-Assisted, Microscopy, Confocal, Prosencephalon, Retina, Signal Transduction, Zebrafish, Zebrafish Proteins, 06 Biological Sciences, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: Development
ISSN: 1477-9129
Language: eng
Dates:
DateEvent
17 November 2015Published
1 October 2015Published Online
21 September 2015Accepted
Publisher License: Creative Commons: Attribution 3.0
Projects:
Project IDFunderFunder ID
089227/Z09/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
G0501487Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
MR/L003775/1Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
G0900994Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
104682/Z/14/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
PubMed ID: 26428010
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/109996
Publisher's version: https://doi.org/10.1242/dev.125120

Actions (login required)

Edit Item Edit Item