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Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology

Annecchino, LA; Morris, AR; Copeland, CS; Agabi, OE; Chadderton, P; Schultz, SR (2017) Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology. Neuron, 95 (5). 1048-1055.e3. ISSN 0896-6273 https://doi.org/10.1016/j.neuron.2017.08.018
SGUL Authors: Copeland, Caroline Susanne

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Abstract

Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed “blind,” meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to target fluorescently labeled neurons. Combining this with robotic automation is difficult, however, as micropipette penetration induces tissue deformation, moving target cells from their initial location. Here we describe a platform for automated two-photon targeted patch-clamp recording, which solves this problem by making use of a closed loop visual servo algorithm. Our system keeps the target cell in focus while iteratively adjusting the pipette approach trajectory to compensate for tissue motion. We demonstrate platform validation with patch-clamp recordings from a variety of cells in the mouse neocortex and cerebellum.

Item Type: Article
Additional Information: © 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Neurology & Neurosurgery, 1109 Neurosciences, 1702 Cognitive Science
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: Neuron
ISSN: 0896-6273
Dates:
DateEvent
30 August 2017Published
11 August 2017Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
BB/K001817/1Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
289146Seventh Framework Programmehttp://dx.doi.org/10.13039/501100004963
2011/R2Royal Societyhttp://dx.doi.org/10.13039/501100000288
URI: http://openaccess.sgul.ac.uk/id/eprint/109995
Publisher's version: https://doi.org/10.1016/j.neuron.2017.08.018

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