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Effective silencing of ENaC by siRNA delivered with epithelial-targeted nanocomplexes in human cystic fibrosis cells and in mouse lung

Tagalakis, AD; Munye, MM; Ivanova, R; Chen, H; Smith, CM; Aldossary, AM; Rosa, LZ; Moulding, D; Barnes, JL; Kafetzis, KN; et al. Tagalakis, AD; Munye, MM; Ivanova, R; Chen, H; Smith, CM; Aldossary, AM; Rosa, LZ; Moulding, D; Barnes, JL; Kafetzis, KN; Jones, SA; Baines, DL; Moss, GWJ; O'Callaghan, C; McAnulty, RJ; Hart, SL (2018) Effective silencing of ENaC by siRNA delivered with epithelial-targeted nanocomplexes in human cystic fibrosis cells and in mouse lung. THORAX, 73 (9). pp. 847-856. ISSN 0040-6376 https://doi.org/10.1136/thoraxjnl-2017-210670
SGUL Authors: Baines, Deborah

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Abstract

Introduction Loss of the cystic fibrosis transmembrane conductance regulator in cystic fibrosis (CF) leads to hyperabsorption of sodium and fluid from the airway due to upregulation of the epithelial sodium channel (ENaC). Thickened mucus and depleted airway surface liquid (ASL) then lead to impaired mucociliary clearance. ENaC regulation is thus a promising target for CF therapy. Our aim was to develop siRNA nanocomplexes that mediate effective silencing of airway epithelial ENaC in vitro and in vivo with functional correction of epithelial ion and fluid transport. Methods We investigated translocation of nanocomplexes through mucus and their transfection efficiency in primary CF epithelial cells grown at air–liquid interface (ALI).Short interfering RNA (SiRNA)-mediated silencing was examined by quantitative RT-PCR and western analysis of ENaC. Transepithelial potential (Vt), short circuit current (Isc), ASL depth and ciliary beat frequency (CBF) were measured for functional analysis. Inflammation was analysed by histological analysis of normal mouse lung tissue sections. Results Nanocomplexes translocated more rapidly than siRNA alone through mucus. Transfections of primary CF epithelial cells with nanocomplexes targeting αENaC siRNA, reduced αENaC and βENaC mRNA by 30%. Transfections reduced Vt, the amiloride-sensitive Isc and mucus protein concentration while increasing ASL depth and CBF to normal levels. A single dose of siRNA in mouse lung silenced ENaC by approximately 30%, which persisted for at least 7 days. Three doses of siRNA increased silencing to approximately 50%. Conclusion Nanoparticle-mediated delivery of ENaCsiRNA to ALI cultures corrected aspects of the mucociliary defect in human CF cells and offers effective delivery and silencing in vivo.

Item Type: Article
Additional Information: © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted. This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/
Keywords: Respiratory System, 1103 Clinical Sciences
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: THORAX
ISSN: 0040-6376
Dates:
DateEvent
September 2018Published
10 May 2018Published Online
9 April 2018Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
WT094348MAWellcome Trusthttp://dx.doi.org/10.13039/100004440
GN2299Action Medical Researchhttp://dx.doi.org/10.13039/501100000317
URI: https://openaccess.sgul.ac.uk/id/eprint/109715
Publisher's version: https://doi.org/10.1136/thoraxjnl-2017-210670

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