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Alga-made anti-Hepatitis B antibody binds to human Fcγ receptors.

Vanier, G; Stelter, S; Vanier, J; Hempel, F; Maier, U; Lerouge, P; Bardor, M; Ma, J (2018) Alga-made anti-Hepatitis B antibody binds to human Fcγ receptors. Biotechnology journal, 13 (4). p. 1700496. ISSN 1860-7314 https://doi.org/10.1002/biot.201700496
SGUL Authors: Ma, Julian

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Abstract

Microalgae are unicellular eukaryotic organisms which represent an emerging alternative to other cell biofactories commonly used to produce monoclonal antibodies. Microalgae display several biotechnological advantages such as their rapid growth rate and their phototrophic lifestyle allowing low production costs as protein expression is solar-fueled. Recently, a fully assembled recombinant IgG antibody directed against Hepatitis B surface antigen is produced and secreted in the culture medium of the diatom Phaeodactylum tricornutum. A biochemical characterization of this recombinant antibody demonstrated that the Asn-297 is N-glycosylated by oligomannosides. In the immune system, antibodies interact with effector molecules and cells through their Fc part and the recognition of Fcγ receptors (FcγR) which are important for inducing phagocytosis of opsonized microbes. Interactions between IgG and FcγR are influenced by the N-glycan structures present on the Asn-297. In this study, the authors characterized the binding capacity of the anti-hepatitis B recombinant IgG produced in P. tricornutum to two human Fcγ receptors (FcγRI and IIIa) using a cellular binding assay and surface plasmon resonance (SPR). This allowed us to demonstrate that the alga-made antibody is able to bind FcγRI with a reduced affinity and engages FcyRIIIa with 3-times higher affinity compared to a control human IgG1.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Vanier, G. , Stelter, S. , Vanier, J. , Hempel, F. , Maier, U. G., Lerouge, P. , Ma, J. and Bardor, M. (2018), Alga‐Made Anti‐Hepatitis B Antibody Binds to Human Fcγ Receptors. Biotechnol. J., 13: 1700496, which has been published in final form at http://doi.org/10.1002/biot.201700496. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: Biotechnology, 1002 Environmental Biotechnology, 1003 Industrial Biotechnology, 1004 Medical Biotechnology
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Biotechnology journal
ISSN: 1860-7314
Dates:
DateEvent
16 April 2018Published
27 December 2017Published Online
27 November 2017Accepted
Publisher License: Publisher's own licence
URI: https://openaccess.sgul.ac.uk/id/eprint/109644
Publisher's version: https://doi.org/10.1002/biot.201700496

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