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Novel domain architectures and functional determinants in atypical annexins revealed by phylogenomic analysis.

Fernandez, M-P; Garcia, M; Martin-Almedina, S; Morgan, RO (2017) Novel domain architectures and functional determinants in atypical annexins revealed by phylogenomic analysis. Biol Chem, 398 (7). pp. 751-763. ISSN 1437-4315 https://doi.org/10.1515/hsz-2016-0273
SGUL Authors: Martin Almedina, Silvia

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Abstract

The fundamental cellular role and molecular interactions of annexins in vesicle trafficking and membrane remodeling remain to be further clarified in order to better understand and exploit their contributions to health and disease. We focused on distinctive features of atypical annexins from all domains of life using phylogenomic, molecular systematic and experimental approaches, to extend the current paradigm and better account for annexin diversity of structure, function and mechanistic role in membrane homeostasis. The analysis of gene duplications, organization of domain architectures and profile hidden Markov models of subfamily orthologs defined conserved structural features relevant to molecular interactions and functional divergence of seven family clades ANXA-G. Single domain annexins of bacteria, including cyanobacteria, were frequently coupled to enzymatic units conceivably related to membrane metabolism and remodeling. Multiple ANX domains (up to 20) and various distinct functional domains were observed in unique annexins. Canonical type 2 calcium binding ligands were well-preserved in roughly half of all ANX domains, but alternative structural motifs comprised of 'KGD', cysteine or tryptophan residues were prominently conserved in the same strategic interhelical loops. Selective evolutionary constraint, site-specific location and co-occurrence in all kingdoms identify alternative modes of fundamental binding interactions for annexins.

Item Type: Article
Additional Information: ©2017 Walter de Gruyter GmbH, Berlin/Boston.
Keywords: 3D modeling, conserved motifs, functional determinants, membrane association, molecular phylogeny, profile hidden Markov models, Amino Acid Motifs, Animals, Annexins, Bacterial Proteins, Evolution, Molecular, Genomics, Humans, MCF-7 Cells, Phylogeny, Protein Domains, 3D modeling, conserved motifs, functional determinants, membrane association, molecular phylogeny, profile hidden Markov models, 3D modeling, conserved motifs, functional determinants, membrane association, molecular phylogeny, profile hidden Markov models, Biochemistry & Molecular Biology, 0601 Biochemistry And Cell Biology
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cell Sciences (INCCCS)
Journal or Publication Title: Biol Chem
ISSN: 1437-4315
Language: eng
Dates:
DateEvent
27 June 2017Published
20 December 2016Published Online
11 December 2016Accepted
Publisher License: Publisher's own licence
Projects:
Project IDFunderFunder ID
BFU2007-67876Ministero de Educacion y CienciaUNSPECIFIED
SV-PA-13-ECOEMP-69Gobierno Principado de AsturiasUNSPECIFIED
GRUPIN14-097Gobierno Principado de AsturiasUNSPECIFIED
PubMed ID: 28002020
Web of Science ID: WOS:000403093600004
Go to PubMed abstract
URI: http://openaccess.sgul.ac.uk/id/eprint/109086
Publisher's version: https://doi.org/10.1515/hsz-2016-0273

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