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Identification of a High Diversity of Cryptosporidium Species Genotypes and Subtypes in a Pediatric Population in Nigeria

Molloy, SF; Smith, HV; Kirwan, P; Nichols, RAB; Asaolu, SO; Connelly, L; Holland, CV (2010) Identification of a High Diversity of Cryptosporidium Species Genotypes and Subtypes in a Pediatric Population in Nigeria. American Journal of Tropical Medicine and Hygiene, 82 (4). pp. 608-613. ISSN 0002-9637 https://doi.org/10.4269/ajtmh.2010.09-0624
SGUL Authors: Molloy, Sile

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Abstract

A longitudinal study was conducted to determine the epidemiology of Cryptosporidium in 1,636 children in Nigeria. Oocyst prevalence ranged from 15.6% to 19.6% over one year. Cryptosporidium hominis (34), C. parvum (25), C. parvum/C. hominis (4), C. meleagridis (5), Cryptosporidium rabbit genotype (5), Cryptosporidium cervine genotype (3), and C. canis (1) were identified by polymerase chain reaction–restriction fragment length polymorphism analysis. Glycoprotein 60 subgenotyping showed that 28 amplifiable C. hominis isolates consisted of 12 subtypes that belonged to 5 subtype families (Ia, Ib, Id, Ie, and 1 novel subtype family, Ih) and 23 amplifiable C. parvum isolates consisted of 6 subtypes that belonged to 4 subtype families (IIa, IIc, Iii, and IIm). Three C. meleagridis isolates sub-genotyped by sequence analysis of the small subunit ribosomal RNA gene fragment were type 1. This study is the first one to genetically characterize Cryptosporidium species and subtypes in Nigeria and highlights the presence of a high Cryptosporidium diversity in this pediatric population.

Item Type: Article
Additional Information: ©The American Society of Tropical Medicine and Hygiene
Keywords: Tropical Medicine, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: American Journal of Tropical Medicine and Hygiene
ISSN: 0002-9637
Dates:
DateEvent
1 April 2010Published
26 March 2010Published Online
26 December 2009Accepted
Publisher License: Publisher's own licence
URI: https://openaccess.sgul.ac.uk/id/eprint/108913
Publisher's version: https://doi.org/10.4269/ajtmh.2010.09-0624

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