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RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6.

Durkin, CH; Leite, F; Cordeiro, JV; Handa, Y; Arakawa, Y; Valderrama, F; Way, M (2017) RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6. Dev Cell, 41 (3). 315-329.e7. ISSN 1878-1551
SGUL Authors: Valderrama, Ferran

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RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing.

Item Type: Article
Additional Information: © 2017 The Francis Crick Institute. Published by Elsevier Inc. This is an open access article under the CC BY license (
Keywords: Pak6, RhoC, RhoD, RhoGTPase crosstalk, Vaccinia virus, blebbing, cell contraction, 06 Biological Sciences, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: Dev Cell
ISSN: 1878-1551
Language: eng
8 May 2017Published
12 April 2017Accepted
Publisher License: Creative Commons: Attribution 4.0
Project IDFunderFunder ID
FC001209Wellcome Trust
FC001209Cancer Research UK
FC001209Medical Research Council
HPMF-CT-2000-01021European Community Marie Curie FellowshipUNSPECIFIED
PubMed ID: 28486133
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