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Single-cell studies of IFN-β promoter activation by wild-type and NS1-defective influenza A viruses.

Killip, MJ; Jackson, D; Pérez-Cidoncha, M; Fodor, E; Randall, RE (2017) Single-cell studies of IFN-β promoter activation by wild-type and NS1-defective influenza A viruses. Journal of General Virology, 98 (3). pp. 357-363. ISSN 0022-1317 https://doi.org/10.1099/jgv.0.000687
SGUL Authors: Perez Cidoncha, Maria Teresa

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Abstract

Deletion or truncation of NS1, the principal IFN antagonist of influenza viruses, leads to increased IFN induction during influenza virus infection. We have studied activation of the IFN induction cascade by both wild-type and NS1-defective viruses at the single-cell level using a cell line expressing GFP under the control of the IFN-β promoter and by examining MxA expression. The IFN-β promoter was not activated in all infected cells even during NS1-defective virus infections. Loss of NS1 expression is therefore insufficient per se to induce IFN in an infected cell, and factors besides NS1 expression status must dictate whether the IFN response is activated. The IFN response was efficiently stimulated in these cells following infection with other viruses; the differential IFN response we observe with influenza viruses is therefore not cell specific but is likely due to differences in the nature of the infecting virus particles and their subsequent replication.

Item Type: Article
Additional Information: ©The Authors. This is an open access article published by the Microbiology Society under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/
Keywords: Green Fluorescent Proteins, Humans, Influenza A virus, Influenza, Human, Interferon-beta, Myxovirus Resistance Proteins, Promoter Regions, Genetic, Single-Cell Analysis, Transcriptional Activation, Viral Nonstructural Proteins, Virus Internalization, Virus Replication, Virology, 06 Biological Sciences, 07 Agricultural And Veterinary Sciences, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Journal of General Virology
ISSN: 0022-1317
Language: eng
Dates:
DateEvent
1 March 2017Published
15 December 2016Published Online
14 December 2016Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
MR/K000241/1Medical Research CouncilUNSPECIFIED
PubMed ID: 27983470
Go to PubMed abstract
URI: http://openaccess.sgul.ac.uk/id/eprint/108556
Publisher's version: https://doi.org/10.1099/jgv.0.000687

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