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Human Gyrovirus Apoptin shows a similar subcellular distribution pattern and apoptosis induction as the chicken anaemia virus derived VP3/Apoptin.

Bullenkamp, J; Cole, D; Malik, F; Alkhatabi, H; Kulasekararaj, A; Odell, EW; Farzaneh, F; Gäken, J; Tavassoli, M (2012) Human Gyrovirus Apoptin shows a similar subcellular distribution pattern and apoptosis induction as the chicken anaemia virus derived VP3/Apoptin. Cell Death and Disease, 3 (e296). ISSN 2041-4889 https://doi.org/10.1038/cddis.2012.34
SGUL Authors: Bullenkamp, Jessica Isabell

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Abstract

The chicken anaemia virus-derived protein Apoptin/VP3 (CAV-Apoptin) has the important ability to induce tumour-selective apoptosis in a variety of human cancer cells. Recently the first human Gyrovirus (HGyV) was isolated from a human skin swab. It shows significant structural and organisational resemblance to CAV and encodes a homologue of CAV-Apoptin/VP3. Using overlapping primers we constructed a synthetic human Gyrovirus Apoptin (HGyV-Apoptin) fused to green fluorescent protein in order to compare its apoptotic function in various human cancer cell lines to CAV-Apoptin. HGyV-Apoptin displayed a similar subcellular expression pattern as observed for CAV-Apoptin, marked by translocation to the nucleus of cancer cells, although it is predominantly located in the cytosol of normal human cells. Furthermore, expression of either HGyV-Apoptin or CAV-Apoptin in several cancer cell lines triggered apoptosis at comparable levels. These findings indicate a potential anti-cancer role for HGyV-Apoptin.

Item Type: Article
Additional Information: ©2012 Macmillan Publishers Limited. Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivative Works 3.0 Unported License.
Keywords: Apoptosis, Apoptosis Regulatory Proteins, Capsid Proteins, Cell Line, Tumor, Cell Nucleus, Cells, Chicken anemia virus, Fibroblasts, Green Fluorescent Proteins, Gyrovirus, HCT116 Cells, Humans, Recombinant Fusion Proteins, Transfection
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cardiac (INCCCA)
Journal or Publication Title: Cell Death and Disease
Article Number: e296
ISSN: 2041-4889
Language: eng
Dates:
DateEvent
April 2012Published
PubMed ID: 22495351
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/107212
Publisher's version: https://doi.org/10.1038/cddis.2012.34

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