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Cug2 is essential for normal mitotic control and CNS development in zebrafish.

Kim, HT; So, JH; Jung, SH; Ahn, DG; Koh, W; Kim, NS; Kim, SH; Lee, S; Kim, CH (2011) Cug2 is essential for normal mitotic control and CNS development in zebrafish. BMC Devopmental Biology, 11 (49). ISSN 1471-213X https://doi.org/10.1186/1471-213X-11-49
SGUL Authors: Kim, Soo-Hyun

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Abstract

Background: We recently identified a novel oncogene, Cancer-upregulated gene 2 (CUG2), which is essential for kinetochore formation and promotes tumorigenesis in mammalian cells. However, the in vivo function of CUG2 has not been studied in animal models. Results: To study the function of CUG2 in vivo, we isolated a zebrafish homologue that is expressed specifically in the proliferating cells of the central nervous system (CNS). Morpholino-mediated knockdown of cug2 resulted in apoptosis throughout the CNS and the development of neurodegenerative phenotypes. In addition, cug2-deficient embryos contained mitotically arrested cells displaying abnormal spindle formation and chromosome misalignment in the neural plate. Conclusions: Therefore, our findings suggest that Cug2 is required for normal mitosis during early neurogenesis and has functions in neuronal cell maintenance, thus demonstrating that the cug2 deficient embryos may provide a model system for human neurodegenerative disorders.

Item Type: Article
Additional Information: © 2011 Kim et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Amino Acid Sequence, Animals, Apoptosis, Cell Transformation, Neoplastic, Central Nervous System, Chromosome Aberrations, Chromosomes, Gene Expression Regulation, Developmental, Kinetochores, Mitosis, Morpholinos, Neurodegenerative Diseases, Neurogenesis, Nuclear Proteins, RNA Interference, RNA, Small Interfering, Sequence Alignment, Spindle Apparatus, Zebrafish, Zebrafish Proteins, Science & Technology, Life Sciences & Biomedicine, Developmental Biology, DEVELOPMENTAL BIOLOGY, DANIO-RERIO, KINETOCHORE, CENTROMERE, DISEASE, CANCER, ORGANIZATION, DISRUPTION, EXPRESSION, COMPLEX, GENES, 06 Biological Sciences, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: BMC Devopmental Biology
ISSN: 1471-213X
Language: eng
Dates:
DateEvent
2011Published
PubMed ID: 21838932
Web of Science ID: WOS:000294900400001
Go to PubMed abstract
URI: http://openaccess.sgul.ac.uk/id/eprint/107211
Publisher's version: https://doi.org/10.1186/1471-213X-11-49

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