SORA

Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

COMPLEMENT-MEDIATED ADIPOCYTE LYSIS BY NEPHRITIC FACTOR SERA

Mathieson, PW; Würzner, R; Oliveria, DB; Lachmann, PJ; Peters, DK (1993) COMPLEMENT-MEDIATED ADIPOCYTE LYSIS BY NEPHRITIC FACTOR SERA. JOURNAL OF EXPERIMENTAL MEDICINE, 177 (6). 1827 - 1831. ISSN 0022-1007 https://doi.org/10.1084/jem.177.6.1827
SGUL Authors: Oliveira, David Benjamin Graeme

[img]
Preview
PDF Published Version
Available under License St George's repository terms & conditions.

Download (404kB) | Preview

Abstract

Recent data indicate a previously unsuspected link between the complement system and adipocyte biology. Murine adipocytes produce key components of the alternative pathway of complement and are able to activate this pathway. This suggested to us an explanation for adipose tissue loss in partial lipodystrophy, a rare human condition usually associated with the immunoglobulin G(IgG) autoantibody nephritic factor (NeF) which leads to enhanced alternative pathway activation in vivo. We hypothesized that in the presence of NeF, there is dysregulated complement activation at the membrane of the adipocyte, leading to adipocyte lysis. Here we show that adipocytes explanted from rat epididymal fat pads are lysed by NeF-containing sera but not by control sera. A similar pattern is seen with IgG fractions of these sera. Adipocyte lysis in the presence of NeF is associated with the generation of fluid-phase terminal complement complexes, the level of which correlates closely with the level of lactate dehydrogenase, a marker of cell lysis. Lysis is abolished by ethylenediaminetetraacetic acid, which chelates divalent cations and prevents complement activation, and reduced by an antibody to factor D, a key component of the alternative pathway. These data provide an explanation for the previously obscure link between NeF and fat cell damage.

Item Type: Article
Additional Information: © 1993 Rockefeller University Press.Beginning six months after publication, RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
Keywords: Adipose Tissue, Animals, Complement C3 Nephritic Factor, Complement Membrane Attack Complex, Complement System Proteins, Humans, L-Lactate Dehydrogenase, Lipodystrophy, Rats, Rats, Inbred BN, Science & Technology, Life Sciences & Biomedicine, Immunology, Medicine, Research & Experimental, Research & Experimental Medicine, IMMUNOLOGY, MEDICINE, RESEARCH & EXPERIMENTAL, ACTIVATION, PATHWAY, ADIPSIN, OBESITY, Immunology, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Clinical Education (INMECE )
Journal or Publication Title: JOURNAL OF EXPERIMENTAL MEDICINE
ISSN: 0022-1007
Related URLs:
Dates:
DateEvent
1 June 1993Published
Web of Science ID: WOS:A1993LD66000036
URI: http://openaccess.sgul.ac.uk/id/eprint/107188
Publisher's version: https://doi.org/10.1084/jem.177.6.1827

Actions (login required)

Edit Item Edit Item