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The pneumococcal response to oxidative stress includes a role for Rgg

Bortoni, ME; Terra, VS; Hinds, J; Andrew, PW; Yesilkaya, H (2009) The pneumococcal response to oxidative stress includes a role for Rgg. MICROBIOLOGY, 155. 4123 - 4134. ISSN 1350-0872 https://doi.org/10.1099/mic.0.028282-0
SGUL Authors: Hinds, Jason

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Abstract

Streptococcus pneumoniae resides in the oxygen-rich environment of the upper respiratory tract, and therefore the ability to survive in the presence of oxygen is an important aspect of its in vivo survival. To investigate how S. pneumoniae adapts to oxygen, we determined the global gene expression profile of the micro-organism in aerobiosis and anaerobiosis. It was found that exposure to aerobiosis elevated the expression of 54 genes, while the expression of 15 genes was downregulated. Notably there were significant changes in putative genome plasticity and hypothetical genes. In addition, increased expression of rgg, a putative transcriptional regulator, was detected. To test the role of Rgg in the pneumococcal oxidative stress response, an isogenic mutant was constructed. It was found that the mutant was sensitive to oxygen and paraquat, but not to H2O2. In addition, the absence of Rgg strongly reduced the biofilm-forming ability of an unencapsulated pneumococcus. Virulence studies showed that the median survival time of mice infected intranasally with the rgg mutant was significantly longer than that of the wild-type-infected group, and the animals infected with the mutant developed septicaemia later than those infected intranasally with the wild-type.

Item Type: Article
Additional Information: Copyright © 2009, SGM This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Aerobiosis, Amino Acid Sequence, Anaerobiosis, Animals, Bacterial Proteins, Base Sequence, Biofilms, DNA Primers, DNA, Bacterial, Female, Gene Expression Profiling, Genes, Bacterial, Mice, Molecular Sequence Data, Mutation, Oxidative Stress, Pneumococcal Infections, Sepsis, Sequence Homology, Amino Acid, Streptococcus pneumoniae, Superoxide Dismutase, Trans-Activators, Virulence, Science & Technology, Life Sciences & Biomedicine, STREPTOCOCCUS-PNEUMONIAE, Microbiology, HYDROGEN-PEROXIDE, GENE-EXPRESSION, CAPSULAR POLYSACCHARIDE, COMPETENCE PHEROMONE, LACTOCOCCUS-LACTIS, PYRUVATE OXIDASE, GENOME SEQUENCE, FENTON REACTION, STRAIN R6, MD Multidisciplinary, Amino Acid Sequence, Aerobiosis
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: MICROBIOLOGY
ISSN: 1350-0872
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Dates:
DateEvent
1 December 2009Published
Web of Science ID: WOS:000272918200032
URI: http://openaccess.sgul.ac.uk/id/eprint/107185
Publisher's version: https://doi.org/10.1099/mic.0.028282-0

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