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Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes

Irving-Rodgers, HF; Morris, S; Collett, RA; Peura, TT; Davy, M; Thompson, JG; Mason, HD; Rodgers, RJ (2009) Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes. HUMAN REPRODUCTION, 24 (4). 936 - 944. ISSN 0268-1161 https://doi.org/10.1093/humrep/den447
SGUL Authors: Mason, Helen Diane

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Abstract

BACKGROUND: The ovarian follicular basal lamina underlies the epithelial membrana granulosa and maintains the avascular intra-follicular compartment. Additional layers of basal lamina occur in a number of pathologies, including pili annulati and diabetes. We previously found additional layers of follicular basal lamina in a significant percentage of healthy bovine follicles. We wished to determine if this phenomenon existed in humans, and if it was related to oocyte function in the bovine. METHODS AND RESULTS: We examined follicles from human ovaries (n = 18) by electron microscopy and found that many follicles had additional layers of basal lamina. Oocytes (n = 222) from bovine follicles with normal or unusual basal laminas were isolated and their ability to undergo in vitro maturation, fertilization and culture to blastocyst was compared. Healthy bovine follicles with a single layer of basal lamina had oocytes with significantly (P < 0.01) greater developmental competence than healthy follicles with additional layers of follicular basal lamina (65% versus 28%). CONCLUSIONS: These findings provide direct evidence that the phenotype of the follicular basal lamina is related to oocyte competence.

Item Type: Article
Additional Information: Copyright: The Authors 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed.
Keywords: Animals, Basement Membrane, Blastocyst, Cattle, Cell Count, Female, Humans, Microscopy, Electron, Transmission, Oocyte Retrieval, Oocytes, Ovarian Follicle, Phenotype, Reproductive Techniques, Assisted, Species Specificity, Science & Technology, Life Sciences & Biomedicine, Obstetrics & Gynecology, Reproductive Biology, OBSTETRICS & GYNECOLOGY, REPRODUCTIVE BIOLOGY, ovary, basal lamina, oocyte competence, in vitro production, BOVINE ANTRAL FOLLICLES, GLYCATION END-PRODUCTS, HUMAN GRANULOSA-CELLS, IN-VITRO MATURATION, BASEMENT-MEMBRANE, EXTRACELLULAR-MATRIX, STIMULATING-HORMONE, CULTURE-CONDITIONS, ATRESIA, GROWTH, 16 Studies In Human Society, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: HUMAN REPRODUCTION
ISSN: 0268-1161
Related URLs:
Dates:
DateEvent
18 December 2009Published
Web of Science ID: WOS:000264391800025
URI: http://openaccess.sgul.ac.uk/id/eprint/107183
Publisher's version: https://doi.org/10.1093/humrep/den447

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