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Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells

Sviderskaya, EV; Easty, DJ; Lawrence, MA; Sanchez, DP; Negulyaev, YA; Patel, RH; Anand, P; Korchev, YE; Bennett, DC (2009) Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells. FASEB JOURNAL, 23 (9). 3179 - 3192. ISSN 0892-6638 https://doi.org/10.1096/fj.08-123596

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Abstract

Stem cells, that is, cells that can both reproduce themselves and differentiate into functional cell types, attract much interest as potential aids to healing and disease therapy. Embryonic neural crest is pluripotent and generates the peripheral nervous system, melanocytes, and some connective tissues. Neural-crest-related stem cells have been reported previously in postnatal skin: committed melanocytic stem cells in the hair follicle, and pluripotent cell types from the hair follicle and papilla that can produce various sets of lineages. Here we describe novel pluripotent neural crest-like stem cells from neonatal mouse epidermis, with different potencies, isolated as 3 independent immortal lines. Using alternative regulatory factors, they could be converted to large numbers of either Schwann precursor cells, pigmented melanocytes, chondrocytes, or functional sensory neurons showing voltage-gated sodium channels. Some of the neurons displayed abundant active TRPV1 and TRPA1 receptors. Such functional neurons have previously been obtained in culture only with difficulty, by explantation. The system was also used to generate comparative gene expression data for the stem cells, melanocytes, and melanoblasts that sufficiently explain the lack of pigment in melanoblasts and provide a rationale for some genes expressed apparently ectopically in melanomas, such as ephrin receptors.—Sviderskaya, E. V., Easty, D. J., Lawrence, M. A., Sánchez, D. P., Negulyaev, Y. A., Patel, R. H., Anand, P., Korchev, Y. E., Bennett, D. C. Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells.

Item Type: Article
Additional Information: Copyright © 2009 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Animals, Cell Differentiation, Cell Line, Cell Separation, Chondrocytes, Gene Expression Profiling, Melanocytes, Mice, Neurons, Pluripotent Stem Cells, Schwann Cells, Skin, Stem Cells, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biology, Cell Biology, Life Sciences & Biomedicine - Other Topics, BIOCHEMISTRY & MOLECULAR BIOLOGY, BIOLOGY, CELL BIOLOGY, determination, differentiation, melanoma, chondrocyte, Schwann cell, FIBROBLAST-GROWTH-FACTOR, ROOT GANGLION NEURONS, MALIGNANT-MELANOMA, MOUSE MELANOCYTES, SENSORY NEURONS, PIGMENT-CELLS, SCHWANN-CELLS, ION-CHANNEL, ADULT SKIN, DIFFERENTIATION, 0601 Biochemistry And Cell Biology, 0606 Physiology, 1116 Medical Physiology
SGUL Research Institute / Research Centre: ?? bio_sci_rc ??
Journal or Publication Title: FASEB JOURNAL
ISSN: 0892-6638
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Dates:
DateEvent
1 September 2009Published
Web of Science ID: WOS:000270241000040
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URI: http://openaccess.sgul.ac.uk/id/eprint/107133
Publisher's version: https://doi.org/10.1096/fj.08-123596

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