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Comparative host specificity of human- and pig- associated Staphylococcus aureus clonal lineages.

Moodley, A; Espinosa-Gongora, C; Nielsen, SS; McCarthy, AJ; Lindsay, JA; Guardabassi, L (2012) Comparative host specificity of human- and pig- associated Staphylococcus aureus clonal lineages. PLOS ONE, 7 (11). e49344. ISSN 1932-6203 https://doi.org/10.1371/journal.pone.0049344
SGUL Authors: Lindsay, Jodi Anne

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Abstract

Bacterial adhesion is a crucial step in colonization of the skin. In this study, we investigated the differential adherence to human and pig corneocytes of six Staphylococcus aureus strains belonging to three human-associated [ST8 (CC8), ST22 (CC22) and ST36(CC30)] and two pig-associated [ST398 (CC398) and ST433(CC30)] clonal lineages, and their colonization potential in the pig host was assessed by in vivo competition experiments. Corneocytes were collected from 11 humans and 21 pigs using D-squame® adhesive discs, and bacterial adherence to corneocytes was quantified by a standardized light microscopy assay. A previously described porcine colonization model was used to assess the potential of the six strains to colonize the pig host. Three pregnant, S. aureus-free sows were inoculated intravaginally shortly before farrowing with different strain mixes [mix 1) human and porcine ST398; mix 2) human ST36 and porcine ST433; and mix 3) human ST8, ST22, ST36 and porcine ST398] and the ability of individual strains to colonize the nasal cavity of newborn piglets was evaluated for 28 days after birth by strain-specific antibiotic selective culture. In the corneocyte assay, the pig-associated ST433 strain and the human-associated ST22 and ST36 strains showed significantly greater adhesion to porcine and human corneocytes, respectively (p<0.0001). In contrast, ST8 and ST398 did not display preferential host binding patterns. In the in vivo competition experiment, ST8 was a better colonizer compared to ST22, ST36, and ST433 prevailed over ST36 in colonizing the newborn piglets. These results are partly in agreement with previous genetic and epidemiological studies indicating the host specificity of ST22, ST36 and ST433 and the broad-host range of ST398. However, our in vitro and in vivo experiments revealed an unexpected ability of ST8 to adhere to porcine corneocytes and persist in the nasal cavity of pigs.

Item Type: Article
Additional Information: PMCID: PMC3498157 ©2012 Moodley et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Animals, Bacterial Adhesion, Cornea, DNA Primers, Female, Host Specificity, Humans, Infectious Disease Transmission, Vertical, Microscopy, Fluorescence, Polymerase Chain Reaction, Pregnancy, Regression Analysis, Staphylococcal Infections, Staphylococcus aureus, Swine, Swine Diseases
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PLOS ONE
ISSN: 1932-6203
Dates:
DateEvent
14 November 2012Published
PubMed ID: 23166643
Web of Science ID: 23166643
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URI: https://openaccess.sgul.ac.uk/id/eprint/101516
Publisher's version: https://doi.org/10.1371/journal.pone.0049344

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