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Failure to activate the IFN-beta promoter by a paramyxovirus lacking an interferon antagonist

Killip, MJ; Young, DF; Ross, CS; Chen, S; Goodbourn, S; Randall, RE (2011) Failure to activate the IFN-beta promoter by a paramyxovirus lacking an interferon antagonist. VIROLOGY, 415 (1). 39 - 46. ISSN 0042-6822
SGUL Authors: Goodbourn, Stephen Edward

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It is generally thought that pathogen-associated molecular patterns (PAMPs) responsible for triggering interferon (IFN) induction are produced during virus replication and, to limit the activation of the IFN response by these PAMPs, viruses encode antagonists of IFN induction. Here we have studied the induction of IFN by parainfluenza virus type 5 (PIV5) at the single-cell level, using a cell line expressing GFP under the control of the IFN-β promoter. We demonstrate that a recombinant PIV5 (termed PIV5-VΔC) that lacks a functional V protein (the viral IFN antagonist) does not activate the IFN-β promoter in the majority of infected cells. We conclude that viral PAMPs capable of activating the IFN induction cascade are not produced or exposed during the normal replication cycle of PIV5, and suggest instead that defective viruses are primarily responsible for inducing IFN during PIV5 infection in this system

Item Type: Article
Additional Information: © 2011 Elsevier Inc. Under a Creative Commons license CC BY 3.0
Keywords: Animals, Cell Line, Cercopithecus aethiops, Defective Viruses, Fluorescent Antibody Technique, Gene Expression Regulation, Green Fluorescent Proteins, Humans, Immunoblotting, Interferon-beta, Mutation, Promoter Regions, Genetic, Rubulavirus, Vero Cells, Viral Proteins, Virus Replication, Science & Technology, Life Sciences & Biomedicine, Virology, Interferon, Interferon antagonist, Paramyxovirus, PIV5, Defective viruses, VESICULAR STOMATITIS-VIRUS, CELL ANTIVIRAL RESPONSES, V-PROTEINS, RIG-I, REGULATORY FACTOR-3, INDUCTION, RNA, SIMIAN-VIRUS-5, PARTICLES, REQUIRES
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: VIROLOGY
ISSN: 0042-6822
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20 June 2011Published
Web of Science ID: WOS:000291296000006
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